Carbon-14 carboxy-labeled polysaccharides

Synthesis of carbon-14 and tritium labeled sagamicin.

Sagamicin, an aminoglycoside antibiotic, was labeled with tritium by means of a platinum catalyzed hydrogen exchange reaction and by carbon-14 in two biosynthetic procedures. 14C-Methyl-L-methionine was used as the radioactive precursor in these biological processes involving Micromonospora sagamiensis. The distribution of radioactivity in 14C-sagamicin and gentamicin C1 was studied by mild aci...

Metabolism of carbon-14-labeled benzene and tolueue in avocado fruit.

The uibiquitouis occturrence of hydrocarbons including mlany aromiatics in plants has been the subject of ain extensive, careful review (2). However. the origin andI metabolisnm of the aromatic hydrocarbons in plaint tissue has received little attention. The intriguilng possible biogenesis of aromatics from the prevalent lpolyacetylenes in plants has been suggested (7). P'reviouis reports from ...

Conversion of Carbon-14-Labeled Urea into Amino Acids in Leaves.

Trapping of DNA-reactive metabolites of therapeutic or carcinogenic agents by carbon-14-labeled synthetic polynucleotides.

Many substances which do not react with DNA directly are metabolized into important DNA-modifying intermediates. We have devised a method for trapping these intermediates with 14C-labeled nucleosides contained in a synthetic polynucleotide. The polynucleotide structure protects the labeled nucleoside from metabolism; thus, it is unaltered when the polymer is incubated with a drug-metabolizing s...

Inflammation and Protein Metabolism Studies of Carbon-14-labeled Proteins in Dogs with Sterile Abscesses

Carbon-14-labeled plasma proteins given by mouth to dogs with sterile abscesses undergo decreased absorption, presumably owing to impaired digestion of protein. The turnover of plasma albumin is greatly accelerated but the globulins, excluding fibrinogen, show little change during the acute stage of the sterile inflammation. Fibrinogen shows very rapid production and utilization during acute in...